Fig. 2

Sac-1004 attenuates IL-1β-induced adhesion of U937 cells to HBMECs. HBMECs were allowed to grow to confluence. Starved cells were treated with Sac-1004 (10 μg/ml, 1 h) followed by IL-1β (10 ng/ml, 6 h). RNA levels of VCAM-1 and ICAM-1 were measured using RT-PCR (a). Protein levels were analyzed using the indicated antibodies (b). HBMECs were treated with IL-1β (10 ng/ml, 6 h) and co-cultured with calcein-AM-labeled monocytes for 1 h. Representative images show the reduction in the IL-1β-induced adhesion of U937 cells to HBMECs (c, d). Attached monocytes were imaged by fluorescent microscopy. The fluorescent intensity of attached monocytes was quantified by a fluorometer at 494 nm (absorbance) and 517 nm (emission). All data are presented as means ± SEM. **P < 0.01