Fig. 3

EAE or administration of IFN-γ overcomes dependence on type I IFN for anti-MOG + C-induced demyelination. a C57BL/6 and IFNAR1-KO mice were immunized with MOG_35–55 as described in “Materials and methods.” At the onset of EAE, animals received either PBS or anti-MOG + C by stereotactic injection into CC. The mice were then monitored for symptoms daily and sacrificed after 2 days. b Graphs showing the quantification of demyelination. Data were analyzed using two-tailed Mann–Whitney U test. Results are shown as mean + SEM, the total number of animals in each group is shown underneath columns. The data represent pooled results from three separate experiments. c Graph shows quantitation of the area of myelin loss. Comparison of first group (IFNAR1-KO alone) with middle group (IFNAR1-KO + rIFNgamma (50 ng)) shows that induction of demyelination by the combination was equivalent to that in wild-type B6 mice (third group ). Statistical significance was assessed using two-tailed Mann–Whitney U test. Results are shown as mean + SEM, the total number of animals in each group is shown underneath columns. The data represent pooled results from at least three separate experiments