Fig. 3

Granzyme B-mediated neurotoxicity. a Pure cortical neuron cultures were treated with granzyme B and the viability and toxicity were measured simultaneously. A single, 24-h exposure to granzyme B at escalating concentrations (4, 8, and 10 nM) replicated a dose-dependent decrease in neuron viability. The 8- and 10-nM doses caused significantly decreased viability relative to control media. **p < 0.01 and ***p < 0.001, respectively, one-way ANOVA with post hoc Dunnett test, n = 8 per condition. Data are shown as the mean ± standard error of the mean from four separate experiments. b When granzyme B was added for five consecutive days, the viability of the all cultures stabilized at day 3. **p < 0.01, ***p < 0.001 and ****p < 0.0001 relative to control media, repeated measures ANOVA with post hoc Dunnett test, n = 10 per condition. Data are shown as the mean ± standard error of the mean from three separate experiments. c Granzyme B-mediated toxicity peaked on day 2 of exposure followed by a decline to control toxicity levels on day 4. ***p < 0.001 and ****p < 0.0001 relative to control media, repeated measures ANOVA post hoc Dunnett test, n = 10 per condition. Data are shown as the mean ± standard error of the mean from three separate experiments