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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Interleukin-1 receptor type 1 is overexpressed in neurons but not in glial cells within the rat superficial spinal dorsal horn in complete Freund adjuvant-induced inflammatory pain

Fig. 5

Localization of IL-1R1 on neurons and glial cells in the superficial spinal dorsal horn of rats. Micrographs of single 1-μmthick laser scanning confocal optical sections illustrating the co-localization between immunolabeling for IL-1R1 (red; ad, e, h, k, n, q) and immunoreactivity for markers that are specific for axon terminals of peptidergic (CGRP, green; a) and non-peptidergic (IB4 binding, green; b) primary afferents, axon terminals of excitatory (VGLUT2, green; c) and inhibitory (VGAT, green; d) intrinsic neurons, astrocytes (GFAP, green; o) and microglial cells (CD11b, green; r), and postsynaptic membranes of excitarory (PSD95, green; i) and inhibitory (gephyrin, green; l) synapses in the superficial spinal dorsal horn. Mixed colors (yellow; marked by white arrowheads) on the superimposed images (j, m, p, s) indicate double-labeled structures. The absence of yellow color on ad indicates a lack of IL-1R1 expression on axon terminals of various origin. IL-1R1 immunoreactive spots appear in two different localization on the micrographs showing immunostaining also for KCC2 (green, f, g): (1) They can be aligned along the lines defined by the KCC2 immunostaining (cell membrane localization; white arrowheads on f and g). (2) They can also be located in areas surrounded by the KCC2-immunostained cell membranes (cytoplasmic localization, yellow arrowhead on g. Bars 2 μm (ad), 5 μm (ns), and 10 μm (em)

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