Fig. 4

Effector memory, central memory, and HSV-1-specific T cells increase and persist in the OB during latency. a Total effector memory (T-EM) and central memory (T-CM) populations were previously gated on CD4⁺ and CD8⁺ T cells (Fig. 3c). Subgates were created to phenotypically characterize T-EM (CD44⁺ CD62L^-) and T-CM (CD44⁺ CD62L⁺). a A representative flow plot for CD4 and CD8 T memory populations is shown from an UI and 60 DPI sample. b, c Graphs of T-EM versus T-CM populations change from UI (n = 4) and 30 to 60 DPI (n = 6). *p < 0.05 and **p < 0.005 as determined by Kruskal-Wallis multiple comparisons test between T-EM or T-CM followed by a Dunn’s multiple comparisons post-test between time points. d Depiction of the frequency of the CD3⁺, CD8⁺ T cells specific for the HSV-1 immunodominant peptide gB, and subdominant peptides RRI, and ICP8 at 30 and 60 DPI. e HSV-1T cell numbers specific for gB represent n = 6–7 for each time point, RRI (30 DPI, n = 4, 60 DPI, n = 10), and ICP8 (30 DPI n = 8, 60 DPI, n = 4)