Fig. 4

P2X7 is necessary for BzATP-induced microglial cell death. Microglia from WT and P2X7^−/− mice were primed with or without 100 ng/ml LPS for 22 h following treatment of 380 μM BzATP for additional 4.5 h. a Representative images of double immunofluorescence for GFAP (green) and Iba1 (red) under indicated conditions. Nuclei were counterstained with DAPI (blue). White box indicates position of high magnification images shown below. Scale bar, 50 μm. b Iba1⁺ microglia quantified by counting at least 3 randomly selected fields under each condition. c Cell viability was calculated by normalizing to vehicle-treated control. Data are shown as mean + SD, n = 3. Experiment was repeated twice independently. One-way ANOVA followed by Tukey’s post hoc test. *P < 0.05; ***P < 0.001