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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: The role of microglial P2X7: modulation of cell death and cytokine release

Fig. 8

AKT and ERK pathways are involved in BzATP-induced P2X7 activation. ac BzATP was applied to microglial culture for 30 min or 1 h in the absence or presence of A-804598. a Western blots were probed with antibodies against p-AKT and p-ERK. b, c Quantification of p-AKT (b) and p-ERK (c) signal intensity normalized to AKT and ERK, respectively. d Microglial cell death was assessed with the treatments of 50 μM U0126 or 1 μM LY294002 in the presence of 10 μM A-804598 for 1 h prior to 380 μM BzATP stimulation for additional 4.5 h. e Microglia were primed by 100 ng/ml LPS for 22 h followed by 50 μM U0126 or 1 μM LY294002 treatment in the presence of 10 μM A-804598 1 h before 380 μM BzATP stimulation for additional 2 h. IL1β release was then conducted by ELISA. Experiment was repeated at least twice independently. One-way ANOVA followed by Tukey’s post hoc test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

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