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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: The Carbon monoxide releasing molecule ALF-186 mediates anti-inflammatory and neuroprotective effects via the soluble guanylate cyclase ß1 in rats’ retinal ganglion cells after ischemia and reperfusion injury

Fig. 7

Effect of ALF-186 treatment on retinal expression of Hsp90 mRNA and protein. a Fold induction of Hsp90 mRNA expression in ischemic retinal tissue compared to GAPDH in relation to the corresponding non-ischemic retinae analyzed by RT-PCR after ALF-186 and sGC inhibition (IRI vs. IRI + ALF-186, *** = p < 0.001; n = 8; data are mean ± SD). b, c Representative Western Blot image and densitometric analysis of n = 8 Western Blots showing the increase of retinal Hsp90 protein compared to β-Actin after ALF-186 (data are mean ± SD; IRI vs. IRI + ALF-186, *** = p < 0.001). This effect was not counteracted by ODQ. d Compared to IRI + PBS, Hsp90 protein expression is increased by ALF-186 treatment, mainly in RGC and bipolar cells. Quantification via frequency density of Hsp90 protein expression in both groups by histogram analysis revealed a significant increase of Hsp90 due to ALF-186 treatment (p < 0.001)

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