Fig. 1

LPS-activated BV2 microglia upregulate Kv1.3 channels. a Immunofluorescence microscopic comparison of Kv1.3 protein expression (detected by Anti-Kv1.3 rabbit pAb 1:500) by unstimulated and LPS-activated (100 ng/mL × 24 h) BV2 microglia. b Western blot confirmation of LPS dose-dependent increase in Kv1.3 protein in BV2 whole-cell lysates (Anti-Kv1.3 pAb 1:1000). c Immunofluorescence microscopic comparison of cell surface Kv1.3 channels in unstimulated and LPS-activated BV2 cells using ShK-F6CA. d Flow cytometric comparison of ShK-F6CA-labeled cell surface Kv1.3 channels and microglial activation marker ICAM-1 in unstimulated and LPS-activated BV2 cells. e Assessment of microglial activation markers MHCII and CD69 in unstimulated and LPS-activated BV2 cells