Fig. 2

In vivo demonstration of Kv1.3 channel upregulation by microglia and brain-infiltrating macrophages in a mouse model of LPS-induced neuroinflammation. a LPS was administered to adult C57BL/6 mice by tail vein injection, and brain mononuclear cells were analyzed by flow cytometry (CD11b, CD45, ShK-F6CA, and ICAM-1). b Most cells were CD11b⁺CD45^low microglia while small populations of brain-infiltrating macrophages CD11b⁺CD45^high and CD11b^- non-myeloid cells were observed. c–e Comparison of Kv1.3 channel and ICAM-1 expression in brain mononuclear cells isolated from control and LPS-treated mice in microglia (c), brain-infiltrating macrophages (d), and CD11b^- subpopulations (e) (*p < 0.05, **p < 0.01, ***p < 0.005)