Fig. 6

LPS-induced MHCI trafficking in microglia involves Kv1.3 channels. a Comparison of MHCI and MHCI-related protein expression in BV2 proteomic data. Normalized LFQ data (compared to control-treatment) is shown (three replicates/group). b Experimental plan for in vivo studies: Adult C57BL/6 mice received four daily IP doses of PBS, ShK-223, LPS, or LPS+ShK-223 and brain mononuclear cells were isolated for flow cytometry (pooled n = 9/group). c Comparison of MHCI (H2Kb) expression (median fluorescence intensity) in CD11b⁺ CD45^low microglia. d Representative flow cytometry frequency histograms of MHCI expression in CD11b⁺ CD45^low microglia. e Comparison of MHCI expression in CD11b⁺CD45^high brain-infiltrating macrophages. f Comparison of the proportions of CD11b⁺CD45^high CNS macrophages (among all CD11b⁺ cells) in the four treatment groups (*p < 0.05, **p < 0.01, ***p < 0.005)