Fig. 3

Effect of IL-1β-treated astrocyte-conditioned media (CM) on microglia gene expression. a Inflammatory gene expression in enriched astrocyte cultures that were treated with PBS or IL-1β (50 ng/mL) for 24 h. n = 5 per group. Data are expressed as mean ± SEM. **p < 0.01, ***p < 0.001 vs. PBS group. b Inflammatory gene expression in primary WT microglia that were treated with PBS-treated astrocyte CM (PBS astrocyte CM) or IL-1β-treated astrocyte CM (IL-1β astrocyte CM) for 4 h. To control for direct effects of IL-1β on primary microglia, empty wells (containing no astrocytes) were treated with PBS or IL-1β for 24 h, and then the media was removed and applied to primary microglia for 4 h. These microglia treatment groups are labeled as PBS and IL-1β, respectively. n = 3 to n = 4 per group. Data are expressed as mean ± SEM. *p < 0.05 vs. PBS astrocyte CM group, #p < 0.05 vs. IL-1β group