Fig. 6

Co-cultured BMEC and SIM-A9 cells respond to luminal endothelial IL-1β treatment. a Occludin (green) immunofluorescence in cultured BMEC. Blue = DAPI staining. Scale bar = 50 μm. b Inflammatory gene expression in BMEC seeded into the upper chambers of transwell plates. PBS or IL-1β (50 ng/mL) was added to the upper chambers (i.e., the luminal surface of the BMEC) for 8 h. Data are representative of two independent experiments. n = 3 to n = 4 per group. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01 vs. PBS group. c Inflammatory gene expression in SIM-A9 cells seeded into the lower chambers of transwell plates containing BMEC in the upper chambers. PBS or IL-1β (50 ng/mL) was added to the upper chambers (i.e., the luminal surface of the BMEC) for 8 h. Wells of SIM-A9 cells without any BMEC in the upper inserts were also treated with PBS or IL-1β. n = 2 to n = 4 per group. Data are expressed as mean ± SEM