Fig. 7

Co-cultured BMEC and SIM-A9 cells respond to abluminal endothelial IL-1β treatment. a Inflammatory gene expression in BMEC seeded into the upper chambers of transwell plates. PBS or IL-1β (50 ng/mL) was added to the lowers chambers (i.e., the abluminal endothelial surface) for 8 h. Data are representative of two independent experiments. n = 3 to n = 4 per group. Data are expressed as mean ± SEM. *p < 0.05 vs. PBS group. b Inflammatory gene expression in SIM-A9 cells seeded into the lower chambers of transwell plates containing BMEC in the upper chambers. PBS or IL-1β (50 ng/mL) was added to the lower chambers (i.e., the abluminal surface of the BMEC) for 8 h. Wells of SIM-A9 cells without any BMEC in the upper inserts were also treated with PBS or IL-1β. n = 2 to n = 3 per group. Data are expressed as mean ± SEM