Fig. 2
NET percentage after incubation with adrenaline, noradrenaline, dexamethasone and acetylcholine. Effect of in vitro administration of 1 × 10−7 M and 1 × 10−5 M adrenaline (a) and noradrenaline (b); 2.5 × 10−7 M and 2.5 × 10−6 M dexamethasone (c); and 5.5 × 10−6 M and 5.5 × 10−4 M acetylcholine (d) on the percentage of NET-producing cells. After incubating blood of healthy donors with hormones and acetylcholine for 4 h at room temperature, neutrophils were isolated, and NETs were induced using PMA or fMLP or left unstimulated (n a,b = 7; n c,d = 6). *p < 0.05; **p < 0.01; ***p < 0.005. The mean and SD ranges are given. For a–c, ANOVA and Bonferroni comparisons as post hoc tests were used. Data in d were assessed by Friedman test and Dunn’s multiple comparison test. Repeated measures tests were used within each of the three stimulation conditions