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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: MHC class II expression and potential antigen-presenting cells in the retina during experimental autoimmune uveitis

Fig. 5

MHC class II expression correlates to EAU severity. Three weeks after adoptive transfer, retinas were carefully dissected, cut into small pieces, and dissociated by incubation with Liberase DL and DNase I at 37 °C for 45 min. Naive eyes were used as control. The single-cell suspensions, excluding dead cells (DAPI+) were analyzed by flow cytometry for MHC II expression using fluorochrome-conjugated specific antibodies. For Figures B to E, data are from 1 representative mouse out of 21 independent mice (5 control, 8 low-score EAU and 8 high-score EAU mice). a Number of MHC class II-expressing cells (normalized per 1 million analyzed retinal cells) for different EAU clinical scores. Data represented: mean ± SEM, ANOVA, and Tukey post-hoc multiple comparisons test, **p < 0.01, ***p < 0.001. b Naive eye with control isotype. c MHC class II expression in the naive retina. d MHC class II expression in the retina during moderate uveitis (clinical grade 1). e MHC class II expression in the retina during severe uveitis (clinical grade 4)

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