Fig. 5From: MHC class II expression and potential antigen-presenting cells in the retina during experimental autoimmune uveitisMHC class II expression correlates to EAU severity. Three weeks after adoptive transfer, retinas were carefully dissected, cut into small pieces, and dissociated by incubation with Liberase DL and DNase I at 37 °C for 45 min. Naive eyes were used as control. The single-cell suspensions, excluding dead cells (DAPI+) were analyzed by flow cytometry for MHC II expression using fluorochrome-conjugated specific antibodies. For Figures B to E, data are from 1 representative mouse out of 21 independent mice (5 control, 8 low-score EAU and 8 high-score EAU mice). a Number of MHC class II-expressing cells (normalized per 1 million analyzed retinal cells) for different EAU clinical scores. Data represented: mean ± SEM, ANOVA, and Tukey post-hoc multiple comparisons test, **p < 0.01, ***p < 0.001. b Naive eye with control isotype. c MHC class II expression in the naive retina. d MHC class II expression in the retina during moderate uveitis (clinical grade 1). e MHC class II expression in the retina during severe uveitis (clinical grade 4)Back to article page