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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: MHC class II expression and potential antigen-presenting cells in the retina during experimental autoimmune uveitis

Fig. 6

MHC class II+ cells upregulate co-stimulatory molecule expression during EAU. Three weeks after adoptive transfer, the retinas were carefully dissected, cut into small pieces, and dissociated by enzymatic digestion. Naive eyes were used as control. The single-cell suspensions, excluding dead cells (DAPI+) were analyzed by flow cytometry for MHC class II, CD40, CD80, and CD86 expression using fluorochrome-conjugated specific antibodies. Data are from one representative mouse (clinical grade 3.75) out of four independent experiments involving eight independent EAU animals. Statistical analysis was performed using t tests, *p value <0.05, **p value <0.01, ***p < 0.001. a Gating on MHC class II+ (red gate) and MHC class IIhi (blue gate) cells. b Expression of co-stimulatory molecules by MHC class II+ cells. c Expression of co-stimulatory molecules by MHC class IIhi cells. d Histogram representation of the mean percentage of co-stimulatory molecule expression by MHC class II+ cells in naive versus EAU retinas. Mean ± SEM (unpaired t test). e Histogram representation of the mean percentage of co-stimulatory molecule expression by MHCIIhi versus MHC class II+ cells during EAU. Mean ± SEM (paired samples t test)

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