Fig. 7

During EAU, both hematopoietic and non-hematopoietic but mostly not endothelial cells are susceptible to express MHC class II. Three weeks after adoptive transfer, the retinas were carefully dissected, cut into small pieces, and dissociated by enzymatic digestion. Naive eyes were used as control. The single-cell suspensions, excluding dead cells (DAPI+) were analyzed by flow cytometry for MHC class II, CD45, CD11b, and CD31 expression using fluorochrome-conjugated-specific antibodies. Data are from one representative mouse out of three independent experiments involving four naive and eight EAU-independent animals. a Naive retina. Left: total retinal cells. Middle: gated on MHC class II⁺ cells (red gate). Right: gated on non-hematopoietic MHC class II⁺ CD45⁻CD11b⁻ cells (green gate). b Adoptive transfer uveitis (clinical grade 3.75). Left: total retinal cells. Middle: gated on MHC class II⁺ cells (red gate). Right: gated on non-hematopoietic MHC class II⁺ CD45⁻CD11b⁻ cells (green gate). c Histogram representation of the mean fluorescence intensity (MFI) for MHC class II expression by CD45⁺CD11b⁺ hematopoietic cells (orange gate) and CD45⁻CD11b⁻ non-hematopoietic cells (green gate) during EAU. Mean ± SEM, Student’s t test, ***p < 0.001