Fig. 2
Concentration–response relationship for protons and steady-state desensitization of ASIC3 with or without the pre-application of PAR2-AP. a The concentration–response curves for protons with or without 10−5 M PAR2-AP pre-application in CHO cells co-expressing ASIC3 and PAR2. Each point represents the mean ± SEM of 8 to 10 neurons. All current values were normalized to the current response induced by pH 6.0 applied alone (marked with asterisk). The curves shown are a best fit of the data to the logistic equation I = I max/[1 + (pH50/pH)n], where pH is the pH value used, I is the normalized current response value, pH50 is the pH value for half-maximal current response, and n is the Hill coefficient. The curves for protons without and with PAR2-AP pre-application were drawn according to the equation described above. b Steady-state desensitization of homomeric ASIC3 expressed in CHO cells with or without PAR2-AP pre-application. PAR2-AP (10−5 M) induced a rightward shift of the pH dependence of steady-state desensitization. Each point represents the mean ± SEM of 6 to 8 neurons. The holding pH varied from 7.6 to 6.6. All currents were induced by pH 6.0 applied alone