Fig. 3

Role of TLR4 in prenatal and postnatal ethanol exposure in PLP, MBP, and Iba-1 immunoreactivity in the cortices of the WT and TLR4-KO pups. Arrows show examples of microglial activation. The scale bar is 50 μm. Bars represent the quantification values of PLP, MBP, and Iba-1 immunoreactivity, expressed as the thresholded area occupied by specific staining in the cortices of the WT and TLR4-KO mice treated, or not, prenatally and postnatally with ethanol (PPEE). Results are given as means ± SEM (n = 5). p < 0.05 compared to the ethanol-treated WT or TLR4-KO mice with their respective untreated control groups