Fig. 4

The electron microscopy analysis shows the role of TLR4 in the synaptic structure alteration in the cerebral cortices of the pups (PND 20) exposed to ethanol. The representative transmission electron micrographs from the cortices of the WT and TLR4-KO pups on PND 20 exposed, or not, to ethanol during the prenatal and postnatal periods are shown. Arrows mark vesicles. PPEE prenatal and postnatal ethanol exposure. Scale bar represents 250 nm. Bars represent the vesicle number and postsynaptic density thickness. Data represent mean ± SEM, n = 5 pups/group. *p < 0.05, compared to their respective control group