Fig. 1

Upregulation of translocator protein 18 kDa (TSPO) expression in the brains of MCAO mice. C57BL/6 mice were subjected to sham operation or 60 min MCAO and reperfusion. Cell suspensions were prepared from whole brain tissues at 24 and 48 h after sham operation or MCAO and reperfusion. a, b Gating strategy for flow cytometry analysis and bar graph show counts of total TSPO⁺ cells in the brain at 24 (a) and 48 h (b) after sham operation or MCAO and reperfusion. n = 6 per group. c, d Gating strategy for flow cytometry analysis and bar graph show counts of CD11b⁺CD45^intTSPO⁺, CD11b⁺CD45^highTSPO⁺, and CD11b⁻CD45^highTSPO⁺ cells in the brain at 24 (c) and 48 h (d) after sham operation or MCAO and reperfusion. n = 6 per group. e, f Immunostaining images and bar graphs show TSPO⁺ and/or Iba1⁺cells in brain slices of mice at 24 (e) and 48 h (f) after sham operation or MCAO and reperfusion. Scale bar: 25 μm, 5 μm (inset). n = 18 sections from six mice in each group. Data were presented as mean ± SEM. *P < 0.05, **P < 0.01