Fig. 5

Treatment with FTY720 does not affect the number of NG2⁺ cells at the level of lateral ventricle. a, b Quantitative analysis of BrdU⁺ and NG2⁺/BrdU⁺ cells. Positive cells were counted using confocal acquired images in the corpus callosum at the level of lateral ventricle (ipsilateral hemisphere). NG2 and BrdU colocalization was determined examining three-dimensional orthogonal reconstruction of confocal layers (ImageJ software). Data are presented as a mean of positive cells per mm² ± SEM and b percentage NG2⁺ cells over BrdU⁺ total cells per mm². Statistical analysis: unpaired t test, two-tailed. N = 4 for each experimental group. c Representative double Z-stack maximal projection of immufluorescence staining for BrdU and NG2 in the corpus callosum (KA + FTY720 group). Scale bar: 50 μm. d Schematic diagram of measured areas in white matter ipsilateral hemisphere (lateral ventricle level)