Fig. 3

2ccPA suppressed the cuprizone-induced acute peak demyelination. Representative photomicrographs of coronal brain sections at the level of the fimbria demonstrate progressive demyelination of the corpus callosum after 5 weeks of cuprizone (CPZ) exposure. Black-Gold II staining in the control (a), CPZ + Ssaline (b), and CPZ + 2ccPA (c) groups. Myelin densities in the corpus callosum (d) were compared with those of controls and expressed as a percentage of the control value using the ImageJ analysis program. Data are mean ± SEM, n = 5 animals. Scale bars = 500 mm. Electron micrographs demonstrate an acute peak demyelination of the corpus callosum after 5 weeks of CPZ exposure. Electron micrographs of control (e), CPZ + Saline (f), and CPZ + 2ccPA (g) in the corpus callosum. Myelinated axons (h), axonal diameter (i), and g-ratio (j). Data are mean ± SEM, n = 7 animals. Statistical analysis was performed using one-way ANOVA followed by post hoc Newman-Keuls test (***p < 0.001 vs. control; ^### p < 0.001 vs. CPZ + Saline). Scale bar = 5 μm