Fig. 7
From: Differential effects of FTY720 on the B cell compartment in a mouse model of multiple sclerosis
In vitro B cell aggregation assay. B cells were purified from naïve B6 mice and pre-incubated with 8 μM FTY720 or corresponding vehicle solution for 90 min. B cells were then stimulated with 0.25 μg/ml LPS and 5 U/ml IL-4 for 72 h to induce aggregation. a Percentage of single cells at the end of the culture period. Graphs show means ± SEM. b, c Representative images of B cell aggregation under each of the two culture conditions. Scale bars represent 100 μm