Fig. 1

Fractalkine prevents bolus H₂O₂-induced demyelination in cerebellar slice cultures. a Experimental timeline shown. b(i) Organotypic cerebellar slices were pre-treated with fractalkine (CX3CL1; 10 ng) for 1 h prior to addition of bolus H₂O₂ (0.5 mM) for 18 h. Graph shows a significant decrease in MBP fluorescence after H₂O₂ treatment. This effect is rescued with CX3CL1 treatment. b(ii) NFH shows no significant decrease with bolus H₂O₂ treatment compared to control. c Images are representative of three separate experiments. Confocal images taken at × 10 magnification. Scale bar, 100 μm. Molecular layer (ML) and white matter tract (WMT) labelled. Data expressed as mean ± SEM; Student’s t test, ^### p < 0.001; and one-way ANOVA and Tukey’s post hoc test, *p < 0.05