Fig. 2
From: The chemokine fractalkine (CX3CL1) attenuates H2O2-induced demyelination in cerebellar slices
Fractalkine prevents bolus H2O2-induced astrocyte cell death in cerebellar slice cultures. Organotypic cerebellar slices were pre-treated with fractalkine (CX3CL1; 10 ng/ml) for 1 h prior to addition of bolus H2O2 (500 μM) for 18 h. a Images are representative of three separate experiments. Confocal images taken at × 10 magnification. Scale bar, 100 μm. Molecular layer (ML) and white matter tract (WMT) labelled. b Graph shows a significant decrease in vimentin fluorescence when treated with bolus H2O2. This effect is rescued when pre-treated with fractalkine. Data expressed as mean ± SEM; Student’s t test, ## p < 0.01; and one-way ANOVA and Tukey’s post hoc test, **p < 0.01