Fig. 4

GOX-CAT-induced reduction in MOG is attenuated by fractalkine. Organotypic cerebellar slices were pre-treated with fractalkine (CX3CL1; 100 ng/ml) for 1 h prior to addition of glucose oxidase (GOX; 1:100,000) and catalase (CAT; 1:500,000). a Images are representative of five separate experiments. Scale bar, 100 μm. Molecular layer (ML) and white matter tract (WMT) labelled. Confocal images taken at × 10 magnification. b(i) Graph shows a significant decrease in MOG fluorescence with GOX-CAT treatment. This effect is rescued with fractalkine treatment at 100 ng/ml. b(ii) NFH shows no significant decrease in fluorescence with GOX-CAT treatment. Graphs expressed as mean ± SEM; Student’s t test, ^### p < 0.001; and one-way ANOVA, *p < 0.05; n = 5