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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Age exacerbates microglial activation, oxidative stress, inflammatory and NOX2 gene expression, and delays functional recovery in a middle-aged rodent model of spinal cord injury

Fig. 2

Microglia and NOX2 component activation and gene expression is altered with age. a Fluorescent microscope images of longitudinal sections of naïve 3- and 12-month-old rat spinal cords. Sections were stained with Iba-1 (green). Sections were imaged at 1.25× magnification and encompass an entire 10-mm section in the thoracic spinal cord. Inserts were taken at 20× magnification in the gray matter delineated by white boxes and arrows. b Immunostaining shows age-related differences in the microglial marker Iba1, showing significantly increased staining in 12- versus 3-month-old rats (size bar = 100 um). N = 4/group. c Comparative qPCR reveals significant increase in gene expression of the pro-inflammatory phenotype marker CD86. d Comparative RT-PCR reveals significant increase in p47PHOX gene expression in 12-month-aged rats compared to 3-month-aged rats. No significant difference was seen in gene expression of p22PHOX or gp91PHOX between the two groups. N = 4/group. *p < 0.05, **p < 0.005, ****p < 0.0005. Bars represent mean ± SEM

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Journal of Neuroinflammation

ISSN: 1742-2094