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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Age exacerbates microglial activation, oxidative stress, inflammatory and NOX2 gene expression, and delays functional recovery in a middle-aged rodent model of spinal cord injury

Fig. 3

ROS production is increased with age. a Fluorescent microscope images of longitudinal sections of naïve 3- and 12-month-old rat spinal cords. Sections were stained with 8-OHdG (green). Sections were imaged at 1.25× magnification and encompass an entire 10-mm long section in the thoracic spinal cord. Inserts were taken at 20× magnification in the gray matter delineated by white boxes and arrows. b 8-OHdG stain shows significantly greater oxidative damage in 12 versus 3-month-old rats (size bar = 300 um). N = 4/group. c Oxyblot analysis shows a trend (p = 0.0946) toward increased protein oxidation in 12-month-aged rats compared to 3-months-aged rats. d Western blot analysis that shows protein expression of 3-NT, a marker of nitrosylated proteins which is a product of oxidative stress, is not significantly different between 12- and 3-month-aged rats. N = 4/group. *p < 0.05. Bars represent mean ± SEM

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Journal of Neuroinflammation

ISSN: 1742-2094