Age exacerbates microglial activation, oxidative stress, inflammatory and NOX2 gene expression, and delays functional recovery in a middle-aged rodent model of spinal cord injury

Table 1 Number of animals per group for all experiments in study

Number of animals per group and experiment
Age	Injured/naïve	Function	Immunohistochemistry	Lesion volume	Biochemistry/PCR
3 months old	Naïve	12	4	0	4
12 months old	Naïve	10	4	0	4
3 months old	Injured	18	4	6	4
12 months old	Injured	17	4	6	4

Animals were first divided by age (3 or 12-months old) and injury type (naïve or injured). Functional analysis was performed on all animals in study. Animals used in function experiments were sacrificed and divided into subgroups for tissue experiments (immunohistochemistry, lesion volume, and biochemistry/PCR)

ISSN: 1742-2094