Age exacerbates microglial activation, oxidative stress, inflammatory and NOX2 gene expression, and delays functional recovery in a middle-aged rodent model of spinal cord injury

Table 3 Comparative gene fold changes for all genes tested by injury group

Comparative fold change by injury group
Naïve		Gene tested	30 dpi
3 months old	12 months old	Gene tested	3 months old	12 months old
1.467 ± 0.8292	**6.993 × 10⁶ ± 1.258 × 10⁶	CD86	1.096 ± 0.2886	***1.253 × 10⁶ ± 145,818
1.371 ± 0.5850	0.7830 ± 0.2167	CD206	1.104 ± 0.3465	**139,991 ± 24,361
2.217 ± 1.785	0.6052 ± 0.04079	p22^PHOX	1.030 ± 0.1825	*20678 ± 6017
8.352 ± 8.037	****39,131 ± 2480	p47^PHOX	7.739 ± 5.672	*1.225 × 10⁶ ± 473,918
0.9453 ± 0.05264	1.583 ± 0.4531	gp91^PHOX	0.9421 ± 0.08263	*1.948 ± 0.4574
3.229 ± 2.839	163.1 ± 141.8	TNFα	0.6954 ± 0.3801	*11.55 ± 2.502
1.226 ± 0.5548	0.8387 ± 0.6209	NFκB	1.027 ± 0.1751	*4.108 ± 0.7734
44.06 ± 22.85	0.01669 ± 0.01419	iNOS	1.109 ± 0.3358	1.069 ± 0.4000
3.118 ± 2.773	1230 ± 794.0	Arg-1	1.047 ± 0.2304	1.382 ± 0.03944
0.4958 ± 0.4531	0.3324 ± 0.02472	CD68	1.104 ± 0.3346	1.011 ± 0.3394
2.438 ± 1.521	7.806 ± 4.643	IL10	1.051 ± 0.2427	1.057 ± 0.1975

Table demonstrates how age affects gene expression before and after injury. The average of the 3-month-old group in both the naïve and injury groups was considered the control mean value. Each individual sample’s fold change was compared to the control mean. Fold changes are presented in table as mean values of all samples in each group ± SEM. N = 4/group
*p < 0.05, **p < 0.005, ***p < 0.0005

ISSN: 1742-2094