Fig. 3

Cuprizone treatment leads to reactive gliosis within the dorsal lateral geniculate nucleus (dLGN). a Bar graph depicting mRNA levels of glial fibrillary acidic protein (Gfap) in the dLGN at 3 (3w) and 5 (5w) weeks of cuprizone treatment. n = 4: Gfap control and cuprizone 3w; n = 3: Gfap control and cuprizone 5w. b Representative confocal images of the dLGN within mouse brain sections immunostained for GFAP at 5 weeks of cuprizone treatment (right panel) or under control conditions (left panel). Note the presence of GFAP-positive astrocytes with hypertrophic morphology (arrows in the right panel). Scale bar: 50 μm. c, d Bar graphs depicting the density of immunostained areas (c) and the number of immuno-positive cells (d) at 5 weeks of cuprizone treatment compared to control conditions (dotted line). Two fields per slice and two slices per animal (n = 4: control; n = 5: cuprizone) were analyzed. All bar graphs depict means ± SEMs. *p < 0.05, **p < 0.01, ***p < 0.001, not significant (ns) p ≥ 0.05 (one-sample t test; compared to set control value = 1)