Fig. 6

Primary cultured rat microglial cells and the effects of SAM and NaHS on cell viability following LPS and ATP stimulation. a Microscope-captured image of microglial cells and b representative photographs of immunofluorescence staining for Iba-1-positive primary cultured rat microglia. c, d The effects of different concentrations of SAM and NaHS on the viability of rat primary cultured microglial cells. LPS and ATP stimulation substantially inhibited cell viability, as demonstrated by CCK-8 assay (p < 0.01 vs. control). These cytotoxic effects were attenuated in a concentration-dependent manner by SAM at concentrations ranging from 50 to 400 μM, and the maximum response was achieved at a concentration of 200 μM (p < 0.01 vs. control). Similar effects were observed with NaHS administration. NaHS exerted its maximal effect at a concentration of 400 μM (p < 0.01 vs. control). N = 3 per group, scale bars = 75 μm. Data are presented as the mean ± SEM. &&p < 0.01 vs. control; &p < 0.05 vs. control; @@p < 0.01 vs. LPS + ATP (with 0 μmol SAM); @p < 0.05 vs. LPS + ATP (with 0 μmol SAM). SAM S-adenosyl-l-methionine, DAPI 4′,6-diamidino-2-phenylindole