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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Endogenous hydrogen sulphide attenuates NLRP3 inflammasome-mediated neuroinflammation by suppressing the P2X7 receptor after intracerebral haemorrhage in rats

Fig. 7

The effects of SAM and NaHS administration on P2X7R expression in primary microglial cells. a Quantitative analysis of P2X7R mRNA levels by qPCR in the control, LPS + ATP, LPS + ATP + SAM and LPS + ATP + NaHS groups. P2X7R mRNAs were evidently elevated after LPS and ATP stimulation (p < 0.01 vs. control) and that both SAM treatment and NaHS treatment significantly suppressed these upregulations (p < 0.05 (a, b)). The relative densities of each mRNA have been normalized against those of the LPS + ATP group. b Representative bands and quantitative analysis of P2X7R in the control, LPS + ATP, LPS + ATP + SAM and LPS + ATP + NaHS groups. P2X7R protein levels were evidently elevated after LPS and ATP stimulation (p < 0.01 vs. control) and that both SAM treatment and NaHS treatment significantly suppressed these upregulations (p < 0.05). The relative densities of each protein have been normalized against those of the LPS + ATP group. c Representative photographs of P2X7R-positive cells in the control, LPS + ATP, LPS + ATP + SAM and LPS + ATP + NaHS groups. The numbers of P2X7R-positive cells were increased and that the cells were visually identical to activated microglia after LPS and ATP stimulation. Both SAM treatment and NaHS treatment significantly alleviated these changes. N = 3 per group, scale bar = 50 μm. Data are the mean ± SEM. **p < 0.01 vs. sham, #p < 0.05 vs. vehicle, &&p < 0.01 vs. control, @p < 0.05 vs. LPS + ATP. SAM S-adenosyl-l-methionine, DAPI 4′,6-diamidino-2-phenylindole

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