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Fig. 10 | Journal of Neuroinflammation

Fig. 10

From: Responses of rat and mouse primary microglia to pro- and anti-inflammatory stimuli: molecular profiles, K+ channels and migration

Fig. 10

Mouse microglia: AgTx-2 sensitive Kv1.3 currents versus activation state. Whole-cell Kv currents were recorded using a modified voltage protocol. From a holding potential of −105 mV, a single step to +45 mV was applied for 1 s before returning to −105 mV for 60 s, and then a voltage ramp was applied from −75 to +45 mV over 120 ms. a Representative traces of Kv currents in the absence and presence of 5 nM AgTx-2 at +45 mV in unstimulated (CTL) cells, and in cells stimulated for 30 h with IFN-γ and TNF-α (I + T), IL-4, or IL-10. For each cell, AgTx-2 was perfused into the bath to record the AgTx-2-insensitive component, which was then subtracted from the total current to yield the Kv1.3 current. b Scatter plot of individual cells showing the proportion of the peak current (at +45 mV) that was blocked by AgTx-2. c Peak current density (pA/pF) as a function of voltage was plotted from the voltage-ramp component: total Kv current (left), AgTx-2 sensitive Kv1.3 current (right). All data are shown as mean ± SEM (number of cells). *p < 0.5; **p < 0.01; ***p < 0.001; ****p < 0.0001

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