Fig. 2

Species comparison of Ptgs2 mRNA and COX-2 protein. Treatments and data presentation are as in Fig. 1. a Ptgs2 mRNA expression (mRNA counts/200 ng total RNA) was determined by NanoString analysis (mean ± SEM; n = 4–6 individual cultures), and plotted on the same Y-axis scale. b Representative Western blots of COX-2 protein. c Summary of fold changes in COX-2 protein expression (mean ± SEM; n = 4–6 individual cultures for mouse and 16 for rat), normalized to the total protein in each lane, and then to control microglia as in Fig. 1. Significant differences from unstimulated (control) cells are indicated: *p < 0.5; **p < 0.01; ***p < 0.001; ****p < 0.0001