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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Responses of rat and mouse primary microglia to pro- and anti-inflammatory stimuli: molecular profiles, K+ channels and migration

Fig. 5

Species comparison of Arg1 mRNA and protein. Treatments and data presentation are as in Fig. 1. a Arg1 mRNA expression (mRNA counts/200 ng total RNA) was determined by NanoString analysis (mean ± SEM; n = 4–6 individual cultures). NB: The Y-axes differ (blue boxes placed for clarity). b Representative Western blots of Arg1 protein. b’. For clarity, to show detection of ARG1 in IL-4-treated rat microglia, an over-exposed blot is shown but was not used for quantification. c Summary of fold changes in Arg1 protein expression (mean ± SEM; n = 4–6 individual cultures for mouse and 21 for rat), normalized to the total protein in each lane, and then to control microglia as in Fig. 1. Again, note that the Y-axis for rat cells is much lower. Significant differences from unstimulated (control) cells are indicated: **p < 0.01; ***p < 0.001; ****p < 0.0001

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