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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Responses of rat and mouse primary microglia to pro- and anti-inflammatory stimuli: molecular profiles, K+ channels and migration

Fig. 6

Species comparison of Aif1 mRNA and Iba1 protein. Treatments and data presentation are as in Fig. 1. a Aif1 mRNA expression (mRNA counts/200 ng total RNA) was determined by NanoString analysis (mean ± SEM; n = 4–6 individual cultures). NB: The Y-axes differ (blue boxes placed for clarity). b Representative Western blots of Iba1 protein. b’ For clarity, to show detection of Iba1 in mouse microglia, an over-exposed blot is shown but was not used for quantification. c Summary of fold changes in Iba1 protein expression (mean ± SEM; n = 3–4 individual cultures for mouse and 14 for rat), normalized to the total protein in each lane, and then to control microglia as in Fig. 1. Here, the Y-axes are the same. Significant differences from unstimulated (control) cells are indicated: *p < 0.5; **p < 0.01; ***p < 0.001; ****p < 0.0001

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