Fig. 9

Rat microglia: AgTx-2 sensitive Kv1.3 currents versus activation state. Whole-cell Kv currents were isolated using a voltage clamp protocol holding at −105 mV, followed by 1-s-long voltage steps from −75 to +45 mV in 20-mV increments, applied every 60 s. a Representative traces of total Kv current in unstimulated (CTL) rat microglia, and in cells stimulated for 30 h with IFN-γ and TNF-α (I + T), IL-4 or IL-10. For each cell, 5 nM AgTx-2 was perfused into the bath to record the AgTx-2 insensitive component, which was then subtracted from the total current to yield Kv1.3 current. b Scatter plot of individual cells showing the proportion of the peak current (at +45 mV) that was blocked by AgTx-2. c Peak current density (pA/pF) as a function of voltages for the total Kv (left) and the AgTx-2-sensitive Kv1.3 (right) currents. Data are shown as mean ± SEM (number of cells). *p < 0.5; **p < 0.01; ***p < 0.001; ****p < 0.0001