Table 1 Rat target sequences used to create Custom CodeSet for nCounter Assay

Aif1

NM_017196.2

ATCGATATTATGTCCTTGAAGCGAATGCTGGAGAAACTTGGGGTTCCCAAGACCCATCTAGAGCTGAAGAAATTAATTAGAGAGGTGTCCAGTGGCTCCG

Arg1

NM_017134.2

ACGGGAAGGTAATCATAAGCCAGAGACTGACTACCTTAAACCACCGAAATAAATGTGAATACATCGCATAAAAGTCATCTGGGGCATCACAGCAAACCGA

Casp1

NM_012762.2

AGATTCTAAGGGAGGACATCCTTTCTCCTCAGAAACAAAAGAAAAACTGAACAAAGAAGGTGGCGCATTTCCTGGACCGAGTGGTTCCCTCAAGTTTTGC

Ccl22

NM_057203.1

TACATCCGTCACCCTCTGCCACCACGTTTCGTGAAGGAGTTCTACTGGACCTCAAAGTCCTGCCGCAAGCCTGGCGTCGTTTTGATAACCATCAAGAACC

Cd163

NM_001107887.1

CCTCTGTAATTTGCTCAGGAAACCAATCGCATACACTGTTGCCATGTAGTTCATCATCTTCGGTCCAAACAACAAGTTCTACCATTGCAAAGGACAGTGA

Cd68

NM_001031638.1

CTCTCATTCCCTTACGGACAGCTTACCTTTGGATTCAAACAGGACCGACATCAGAGCCACAGTACAGTCTACCTTAACTACATGGCAGTGGAATACAATG

Cx3cr1

NM_133534.1

ATGTGCAAGCTCACGACTGCTTTCTTCTTCATTGGCTTCTTTGGGGGCATATTCTTCATCACCGTCATCAGCATCGACCGGTACCTCGCCATCGTCCTGG

Cybb

NM_023965.1

CAGTACCAAAGTTTGCCGGAAACCCTCCTATGACTTGGAAATGGATCGTGGGTCCCATGTTCCTGTATCTGTGTGAGAGGCTGGTGCGGTTTTGGCGATC

Fcgr1a

NM_001100836.1

TGATGGATCATACTGGTGCGAGGTAGCCACGGAGGACGGCCGTGTCCTTAAGCGCAGCACCAAGTTGGAGCTATTTGGTCCCCAGTCATCAGATCCTGTC

Fcgr2b

NM_175756.1

CTGGTCCAAGGAATGCTGTAGATATGAAAGAAAACATCTAGAGTCCCTTCTGTGAGTCCTGAAACCAACAGACACTACGATATTGGTTCCCAATGGTTGA

Fcgr3a

NM_207603.1

GACTCTTGTTTGCAATAGACACAGTGCTGTATTTCTCGGTGCAGAGGAGTCTTCAAAGTTCCGTGGCAGTCTATGAGGAACCCAAACTTCACTGGAGCAA

Gusb

NM_017015.2

TCATTTGATCCTGGATGAGAAACGAAAAGAATATGTCATCGGAGAGCTCATCTGGAATTTTGCTGACTTCATGACGAACCAGTCACCACTGAGAGTAACA

Hprt1

NM_012583.2

AGCTTCCTCCTCAGACCGCTTTTCCCGCGAGCCGACCGGTTCTGTCATGTCGACCCTCAGTCCCAGCGTCGTGATTAGTGATGATGAACCAGGTTATGAC

Ifng

NM_138880.2

AAGGACGGTAACACGAAAATACTTGAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTTGAAAGACAACCAGGCCATCAGCAACAACATAA

Ifngr1

NM_053783.1

CCTGTTACACATTCGACTACACTGTGTTTGTGAAACATTACAGGAGTGGGGAGATCCTACATACAGAACATAGCGTCCTAAAAGAAGATTGTAGCGAAAC

Ifngr2

NM_001108313.1

TTTCTTAAGTTACACTTAGTAAAGCAGATGAGTCCGCAGGAGACTTCAGCAAGAAAGAAGTTCCTACCGTCTCATCCCTTAGTTCTTCAAAGCCAAAGGA

Il10

NM_012854.2

ACAACATACTGCTGACAGATTCCTTACTGCAGGACTTTAAGGGTTACTTGGGTTGCCAAGCCTTGTCAGAAATGATCAAGTTTTACCTGGTAGAAGTGAT

Il10ra

NM_057193.2

TGTTTACATGTCACGACGGAGCATTATTTCACCGTGACCAACCTCAGCATTTTCTTCTTATCCATCCTGATACTCTGTGGAGCCCTGGTCTGCCTGGTTC

Il10rb

NM_001107111.1

CCTCCCTGGATCGTGGCCATCATCCTTATAGCCTCCGTCTTGATAGTCTTCCTCTTCCTACTGGGCTGCTTCAGCATGGTGTGGTTCATTTACAAGAAGA

Il13ra1

NM_145789.2

TAACGAATTTGAGTGTCTCTGTCGAAAATCTCTGCACAATAGTGTGGACATGGAGTCCTCCTGAGGGAGCCAGTCCAAATTGCAGTCTCAGATATTTTAG

Il1b

NM_031512.1

TGCACTGCAGGCTTCGAGATGAACAACAAAAATGCCTCGTGCTGTCTGACCCATGTGAGCTGAAAGCTCTCCACCTCAATGGACAGAACATAAGCCAACA

Il1r1

NM_013123.3

CTCATATTCTGGAGACTGCACACGTACGGTTAGTATACCCAGTTCCTGACTTCAAGAATTACCTCATCGGGGGCTTTGCCATCTTCACAGCTACAGCCGT

Il1rn

NM_022194.2

TCATTGCTGGGTACTTACAAGGACCAAATACCAAACTAGAAGAAAAGATAGACATGGTGCCTATTGACTTTCGGAATGTGTTCTTGGGCATCCACGGGGG

Il4

NM_201270.1

TGCTGTCACCCTGTTCTGCTTTCTCATATGTACCGGGAACGGTATCCACGGATGTAACGACAGCCCTCTGAGAGAGATCATCAACACTTTGAACCAGGTC

IL4r

NM_133380.2

GGGTGTCAGCATCTCCTGCATCTGCATCCTATTGTTTTGCCTGACCTGTTACTTCAGCATTATCAAGATTAAGAAGATATGGTGGGACCAGATTCCCACT

Il6

NM_012589.1

GGAACAGCTATGAAGTTTCTCTCCGCAAGAGACTTCCAGCCAGTTGCCTTCTTGGGACTGATGTTGTTGACAGCCACTGCCTTCCCTACTTCACAAGTCC

Itgam

NM_012711.1

CATCCCTTCCTTCAACAGTAAAGAAATATTCAACGTCACCCTCCAGGGCAATCTGCTATTTGACTGGTACATCGAGACTTCTCATGACCACCTCCTGCTT

Kcna2

NM_012970.3

GCCGGCCAGGATCATAGCCATTGTATCTGTGATGGTCATTCTGATCTCGATCGTCAGCTTCTGTCTGGAAACCTTGCCCATCTTCCGGGATGAGAACGAG

Kcna3

NM_019270.3

GCCACCTTCTCCAGAAATATCATGAACCTGATAGACATTGTAGCCATCATCCCTTATTTTATTACTCTGGGCACTGAGCTGGCTGAGCGACAGGGTAATG

Kcna5

NM_012972.1

ATCAGAAGGGGTAGCTGTCCTCTAGAAAAGTGTCACCTCAAGGCCAAGAGCAACGTGGACTTGCGGAGGTCCCTGTATGCCCTCTGTCTGGACACTAGCC

Kcnj2

NM_017296.1

GTTCTTTGGCTGTGTGTTTTGGTTGATAGCTCTGCTCCACGGGGATCTGGATGCTTCTAAAGAGAGCAAAGCGTGTGTGTCTGAGGTCAACAGCTTCACG

Mrc1

NM_001106123.1

CTTTGGAATCAAGGGCACAGAGCTATATTTTAACTATGGCAACAGGCAAGAAAAGAATATCAAGCTTTACAAAGGTTCCGGTTTGTGGAGCAGATGGAAG

Msr1

NM_001191939.1

CACGTTCCATGACAGCATCCCTTCCTCACAACACTATAAATGGCTCCTCCGTTCAGGAGAAACTGAAGTCCTTCAAAGTTGCCCTCGTCGCTCTCTACCT

Myc

NM_012603.2

ACCGAGGAAAACGACAAGAGGCGGACACACAACGTCTTGGAACGTCAGAGGAGAAACGAGCTGAAGCGTAGCTTTTTTGCCCTGCGCGACCAGATCCCTG

Ncf1

NM_053734.2

TCCATTCCCAGCATCCCATAATTGGGCTTGTCCGTGTTCCAACATCTGGGCGGAATTTCACAGCCAAAGGTCAAGAGGACTGCTGTTACGTTCAAGGTCG

Nfkbia

NM_001105720.2

TATTGTGCTTTTGGTTGAACCGCCATAGACTGTAGCTGACCCCAGTGTGCCCTCTCACGTAAGAACCAGGTGTTCAGTGGTATGTGCTTAAGTCATCCCC

Nos2

NM_012611.2

ACGGGACACAGTGTCGCTGGTTTGAAACTTCTCAGCCACCTTGGTGAGGGGACTGGACTTTTAGAGACGCTTCTGAGGTTCCTCAGGCTTGGGTCTTGTT

Nox1

NM_053683.1

CCGAGAAAGAAGATTCTTGGCTAAATCCCATCCAGTCTCCAAACGTGACAGTGATGTATGCAGCATTTACCAGTATTGCTGGCCTTACTGGAGTGGTCGC

Nox4

NM_053524.1

TGTTGGACAAAAGCAAGACTCTACATATCACCTGTGGCATAACTATTTGTATTTTCTCAGGTGTGCATGTAGCTGCCCACTTGGTGAACGCCCTGAACTT

Nr3c1

NM_012576.2

AGCTTTCCTTGAAGCGTATAAAGAGCCATGCTCCTTTAGTATGTGGGGAAGAAGAGAGCTGTCATAGTTTTGAGTACAGTGAGAAGATGCGGTACTGTCT

P2rx7

NM_019256.1

ACTTTAAGAGGTCACATTAACCAGACTAGAAGCCATCGCATCTAACCGCATACCAGACACAGTCTGACGCCTCATTGCTATGCTATGGTTCTAAGTGACT

P2ry12

NM_022800.1

TGATAACCATTGACCGATACCTGAAGACCACCAGACCATTTAAAACTTCCAGCCCCAGCAATCTTTTGGGTGCGAAGATTCTTTCTGTTGCCATCTGGGC

P2ry2

NM_017255.1

GAGCTCTTTAGCCATTTTGTGGCTTACAGCTCTGTCATGCTGGGTCTGCTTTTTGCTGTGCCCTTTTCCATCATCCTGGTCTGTTACGTGCTCATGGCCC

Pparg

NM_013124.1

TTTATAGCTGTCATTATTCTCAGTGGAGACCGCCCAGGCTTGCTGAACGTGAAGCCCATCGAGGACATCCAAGACAACCTGCTGCAGGCCCTGGAACTCC

Ptgs2

NM_017232.3

TTCGGAGGAGAAGTGGGTTTTAGGATCATCAACACTGCCTCAATTCAGTCTCTCATCTGCAATAATGTGAAAGGGTGTCCCTTTGCCTCTTTCAATGTGC

Ptk2b

NM_017318.2

GCAGTGATCATGAAGAATCTTGACCACCCTCACATCGTCAAGCTGATTGGCATCATTGAAGAGGAACCCACATGGATCGTCATGGAACTGTATCCTTATG

Ptpn6

NM_053908.1

GCAGAGTCACTGCTGCAGGCCAAGGGCGAGCCCTGGACATTTCTTGTGCGTGAGAGTCTCAGCCAACCTGGTGATTTTGTGCTCTCTGTGCTCAATGACC

Retnla

NM_053333.1

AGGAACTTCTAGCCCATCAAGATAACTATCCCTCTGCTGTAAGGAAGACCCTCTCATGCACTAATGTCAAGTCTATGAGCAAATGGGCCTCCTGCCCTGC

Socs1

NM_145879.1

CGGCCGCTGCAGGAGCTGTGTCGCCAGCGCATCGTGGCCGCCGTGGGTCGCGAGAACCTGGCACGCATCCCTCTTAACCCGGTACTCCGTGACTACCTGA

Socs3

NM_053565.1

GGAAGACTGTCAACGGTCACCTGGACTCCTATGAGAAAGTGACCCAGCTGCCTGGACCCATTCGGGAGTTCCTGGACCAGTATGATGCTCCACTTTAAAG

Tgfb1

NM_021578.2

CGCCTGCAGAGATTCAAGTCAACTGTGGAGCAACACGTAGAACTCTACCAGAAATATAGCAACAATTCCTGGCGTTACCTTGGTAACCGGCTGCTGACCC

Tgfbr1

NM_012775.2

GTCTGCATTGCACTTATGCTGATGGTCTATATCTGCCATAACCGCACTGTCATTCACCACCGCGTACCAAATGAAGAGGATCCCTCACTAGATCGCCCTT

Tgfbr2

NM_031132.3

CCAGCAGTCCTGACCTGTTGCTGGTCATTATCCAAGTGACGGGCGTCAGCCTCCTGCCTCCGCTGGGGATTGCCATAGCTGTCATTGCCATCTTCTACTG

Tlr2

NM_198769.2

TTTACAAACCCTTAGGGTAGGAAATGTTGACACTTTCAGTGAGATAAGGAGAATAGATTTTGCTGGGCTGACCTCTCTCAACGAACTTGAAATTCAGGTA

Tlr4

NM_019178.1

GTCAGTGTGCTTGTGGTAGCCACTGTAGCATTTCTGATATACCACTTCTATTTTCACCTGATACTTATTGCTGGCTGTAAAAAGTACAGCAGAGGAGAAA

Tnf

NM_012675.2

GGTGATCGGTCCCAACAAGGAGGAGAAGTTCCCAAATGGGCTCCCTCTCATCAGTTCCATGGCCCAGACCCTCACACTCAGATCATCTTCTCAAAACTCG

Tnfrsf1a

NM_013091.1

TATTCTTTATCTGCATCAGTCTACTGTGCCGATATCCCCAGTGGAGGCCCAGGGTCTACTCCATCATTTGTAGGGATTCAGCTCCTGTCAAAGAGGTGGA

Tnfrsf1b

NM_130426.4

AGGAGTTCAGATTCTTCCCATGGCAGCCACGGGACCCATGTCAACGTCACCTGCATCGTGAACGTCTGTAGCAGCTCTGACCACAGCTCTCAGTGTTCTT

Trem2

NM_001106884.1

TCCGGCTGGCTGAGGAAGGGTGCCATGGAACCTCTCCACGTGTTTGTCCTGTTGCTGGTCACAGAGCTGTCCCAAGCCCTCAACACCACAGTGCTGCAGG

Tspo

NM_012515.1

GCTGCCCGCTTGCTGTATCCTTACCTGGCCTGGCTGGCCTTTGCCACCATGCTCAACTACTATGTATGGCGTGATAACTCTGGTCGGCGAGGGGGCTCCC