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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Neuroinflammation contributes to autophagy flux blockage in the neurons of rostral ventrolateral medulla in stress-induced hypertension rats

Fig. 5

LC3 expression analysis. a Representative gels (inset) or densitometric analysis of ratio between light chain 3 (LC3)-II and LC3-I protein level in different groups. b Representative immunofluorescent photomicrographs for LC3B. c Qualitative analysis of the number of LC3B detected from RVLM neurons of SIH or normotensive rats. d Representative photomicrographs of LC3 dots in rats when the GFP-LC3 adeno-associated virus was transfected into the RVLM, implying the enhancement of autophagosomes in SIH. Values are mean ± SEM of quadruplicate analyses on samples pooled from five to six animals in each group. *P < 0.05 vs. control rats in the post hoc Scheffé multiple range analysis. RVLM rostral ventrolateral medulla. Scale bar, 20 μm in b and 40 μm in d

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