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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Arginine vasopressin altered the expression of monocarboxylate transporters in cultured astrocytes isolated from stroke-prone spontaneously hypertensive rats and congenic SHRpch1_18 rats

Fig. 4

Effect of AVP during hypoxia and reoxygenation (H/R) on Slc16a1 and Slc16a4 gene expression in astrocytes isolated from WKY/Izm and SHRpch1_18 rats. Confluent astrocytes isolated from WKY/Izm and SHRpch1_18 rats were exposed to hypoxic (1% O2) conditions for 24 h with or without 100 nM AVP. After hypoxic culture, they were maintained under normoxic conditions for 30 min or for 2 h with or without 100 nM AVP. Total cellular RNA was isolated from the cultured astrocytes for RT-PCR, using primers for Slc16a1 or Slc16a4. a. Slc16a1 and b. Slc16a4 gene expression from astrocytes from all assayed rat strains. Columns show means ± SE (n = 4). Re30, after hypoxic culture (1% O2), cells were maintained in normoxic conditions (21% O2) for 30 min; Re2h, after hypoxic culture (1% O2), cells were maintained in normoxic conditions (21% O2) for 2 h. AVP 100 nM arginine vasopressin. *P < 0.05

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