Skip to main content
Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Priming astrocytes with TNF enhances their susceptibility to Trypanosoma cruzi infection and creates a self-sustaining inflammatory milieu

Fig. 2

TNF pre-treatment enhances the infection of astrocyte cultures by Trypanosoma cruzi. a C57BL/6 primary astrocyte cell cultures were pre-treated with rTNF, infected at MOIs of 1:1 and 10:1, and analyzed 4 h postinfection. Giemsa staining revealed intracellular forms of T. cruzi (arrows) in untreated (NT) and TNF-treated infected astrocytes. b Astrocyte cultures were pre-treated with rTNF (0.1, 1, 10 ng/mL final concentrations) and infected with T. cruzi (MOI 1:1, 4 h), and the number of parasites per 100 cells and the percentage of infected cells were analyzed. c Number of parasites per 100 cells and the percentage of infected cells were analyzed after infection at MOIs of 1:1 and 10:1 and pre-treatment with rTNF at 1 ng/mL. Data are presented as the means ± SEM of triplicates. * p < 0.05 and ** p < 0.01 for untreated (NT) vs. rTNF pre-treated astrocytes. # p < 0.05 and ## p < 0.01 MOI of 1:1 vs. MOI of 10:1

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094