Fig. 5

Glibenclamide does not alter circulating or splenic immune cell counts. Leukocyte suspensions were prepared from the blood or spleen of control mice or mice after 3 days of treatment with fingolimod (Fin; 3 mg/kg by gavage daily) or glibenclamide (Glib; 10 μg/mouse IP daily). a, b Representative plots (a) and quantification b showing the percent of total CD45+ leukocytes, and CD45 + CD4+ and CD45 + CD8+ T-lymphocytes in the three treatment groups, with respect to the control group, determined by FACS. c Representative plot and quantification showing the percent of total CD3+ cells with respect to the control group, and the ratios of CD3 + CD4+ to CD3 + CD8+ cells in the three treatment groups, determined by FACS; 3 mice/group; *P < 0.05 and **P < 0.01 with respect to control (CTR)