Fig. 3
Combined high-fat and high-salt diet has no additional effect on EAE severity and immune cell activation. a Clinical course of MOG35–55 EAE. Mice were fed either a NaCl-rich or a NaCl + LA-rich diet for 4 weeks prior to immunization. Data are shown on a 5-point score scale (n = 15–20 mice per group, data pooled from four independent experiments, *p < 0.05). b, c Ex vivo flow cytometry analysis of Th1 (b) and Th17 (c) cell frequencies in the spinal cord under a NaCl-rich or NaCl + LA-rich diet versus the control diet on day 21 of MOG35–55 EAE (n = 9–13 mice per group, data pooled from three independent experiments, *p < 0.05). d–f Ex vivo flow cytometry analysis of Th1 (d), Th17 (e), and Treg (f) cell frequencies in the spleen under a NaCl-rich or NaCl + LA-rich diet versus the control diet on day 10 of MOG35–55 EAE (n = 4–5 mice per group, *p < 0.05, **p < 0.01). g, h Cytokine analyses in splenocyte culture after ex vivo recall with MOG35–55 (splenocytes harvested on day 10 p.i. of MOG35–55 EAE, n = 4–5 per group, *p < 0.05, **p < 0.01, ***p < 0.001)