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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Tetrahydrobiopterin (BH4) deficiency is associated with augmented inflammation and microvascular degeneration in the retina

Fig. 3

Microvascular degeneration in the retina of the tetrahydrobiopterin (BH4) deficient mice is associated with an augmentation on activated microglial cells with age. Representative confocal images from retinas showing immunoreactivity to TRITC-conjugated lectin endothelial cell marker Bandeiraea simplicifolia (red) and microglial marker Iba-1 (green) in the superficial (ad) and deep (c, d) retinal vascular complexes from controls (WT) and hph-1 mice at different postnatal ages (P1, P7, P14, and P22). Merged images show co-localization in yellow. d Iba-1 immunopositive cells were significantly increased in the superficial and deep retinal vascular complexes and associated with a dramatic absence of the deep microvasculature in hph-1 mice at 22-days-old. Histogram (ad) compiles average number of Iba-1-positive cells per mm2 quantified in three different fields on the superficial and/or deep vascular complexes for each group (n = 4–5 per group). ns not significant, *p < 0.05, **p < 0.01 vs control. Scale bar = 100 μm

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