Fig. 5

Thrombospondin-1 (TSP-1) is localized in microglial cells and its receptor CD36 in the retinal microvasculature from BH4 deficient mice. a Representative confocal images from retinal flatmounts showing immunoreactivity of TSP-1 (red) and Iba-1 (green). Co-staining of TSP-1 with Iba-1 was detected mainly on activated microglial cells (yellow) localized in the nerve fiber layer (NFL) or the ganglion cell layer (GCL) in retinas from hph-1 mice but not in the control (WT). Scale bar = 50 μm. b Representative confocal images of CD36 receptor (green) and lectin (red) merged with DAPI (blue and yellow) in retinal cryosections from control and hph-1 mice at 22-day-old (n = 3 per group). CD36 receptor staining was highly detected in the damaged microvasculature present in hph-1 mice (yellow), but not in the WT mice. Scale bar = 50 μm