Fig. 7

Antiangiogenic effect of hyperoxic microglia-conditioned media containing TSP-1 is abolished by the immunoneutralization of TSP-1 or with pre-incubation of microglial cells with BH4. a Representative micrographs of choroidal explants stimulated with conditioned media (CM) from microglial cells (MG) previously exposed to normoxia (Nor-MGCM) or hyperoxia stimulated with vehicle (Hyp-MGCM + Veh) or BH4 (Hyp-MGCM + BH4) and/or in presence of a TSP-1 neutralizing monoclonal antibody (1.2 μg/ml, Hyp-MGCM + anti-TSP-1) for 48 h. Choroidal explants cultured in matrigel containing growth factors and treated with Nor-MGCM displayed increased sprouting, whereas explants exposed to Hyp-MGCM + Veh exhibited a significant diminished sprouting growth. The choroidal explants incubated with Hyp-MGCM + BH4 or in presence of a TSP-1 neutralizing antibody showed a significant augmentation in the sprouting compared to Hyp-MGCM + Veh. b The histogram depicts the quantification of choroidal explant sprouting (^# p < 0.0001 compared to Nor-MGCM, ^& p < 0.05, and *p < 0.001 compared to Hyp-MGCM + Veh; n = 5 per group)