Fig. 4
From: Inhibition of hypothalamic leukemia inhibitory factor exacerbates diet-induced obesity phenotype
Impact of inhibiting hypothalamic LIF on inflammatory markers. a Schematic representation of the protocol employed to immunoneutralize hypothalamic LIF. b The expression of LIF protein was evaluated by immunoblot in the hypothalamus of obesity-resistant (OR) mice treated with non-immune IgG or anti-LIF antibody for 15 days. Real-time PCR was employed to determine the relative expression of transcripts encoding for TNF-α (c), IL-1β (d), CX3CL1 (fractalkine) (e), cxcl1 (f) IL-6 (g), and IL-10 (h) ccl20 (i) in the hypothalamus of obesity-prone (OP) mice treated with non-immune IgG or OR mice treated either with non-immune IgG or anti-LIF antibody. In b–g, n = 5; *p < 0.05 vs. OP IgG and §p < 0.05 vs. OR IgG